abstract：:Various drugs added to the culture medium of rabbit mammary gland were assayed for their capacity to affect the lactogenic and the mammogenic activities of prolactin. Three lysosomotropic agents NH4Cl, chloroquine and methylamine which were previously demonstrated to inhibit the degradation of the hormone-receptor com...

journal_title：European journal of cell biology

authors： Houdebine LM

更新日期：1980-10-01 00:00:00

abstract：:We have previously demonstrated the immunofluorescent localization of the tau and HMW MAPS on microtubules in vivo. When cells were treated with the non-ioni detergent triton before fixation, however treated with the non-ionic detergent triton before fixation, however, the immunofluorescent staining of microtubules wi...

journal_title：European journal of cell biology

authors： Connolly JA,Kalnins VI

更新日期：1980-08-01 00:00:00

abstract：:HeLa cells in mitosis are fixed by KMnO4 to obtain a good preservation of membranes. Inside and around the mitotic apparatus a vesicular, a tubular and a cisternal type of membranes are demonstrated. Small spherical or ellipsoidal vesicles are distributed at random in the spindle area. Zones around the poles and in th...

journal_title：European journal of cell biology

authors： Moll E,Paweletz N

更新日期：1980-08-01 00:00:00

abstract：:A new, two step method is described for the isolation of megakaryocytes from bone marrow. By means of centrifugation in a PercollR density gradient megakaryocytes can be enriched by factor 68 or 24 starting from rat or guinea pig bone marrow, respectively. The purity of the megakaryocytes can be improved ca. 4 fold if...

journal_title：European journal of cell biology

authors： Seitz R,Wesemann W

更新日期：1980-06-01 00:00:00

abstract：:The exhaustion of metabolically poisoned muscle fibres is accompanied by a one hundred-fold increase in potassium conductance. Under these conditions a normal membrane capacity was measured. Extracellular markers (lanthanum, ferritin) were traced in the transverse tubular system but not in the myoplasm or the sarcopla...

journal_title：European journal of cell biology

authors： Fink R,Grocki K,Lüttgau HC

更新日期：1980-04-01 00:00:00

abstract：:Hydrogencarbonate and chloride activated, ouabain-insensitive ATPase activities are demonstrated in the salt-absorbing rectum of larval dragonflies. Maximal activation is achieved at approx. 30 mM HCO3- and 20 mM Cl-, respectively. The stimulation of each anion obeys Michaelis-Menten kinetics Km values are 4.65 mM for...

journal_title：European journal of cell biology

authors： Komnick H,Schmitz M,Hinssen H

更新日期：1980-02-01 00:00:00

abstract：:Perfused Chara cells capable of resuming ATP-dependent cytoplasmic streaming in low free Ca++ solutions have been examined by electron microscopy for myosin-like filaments. Filaments 44 nm in diameter and up to 3 micron in length have been found associated with the endoplasmic reticulum that along with mitochondria, m...

journal_title：European journal of cell biology

authors： Williamson RE

更新日期：1979-12-01 00:00:00

abstract：:Application of carbon tetrachloride produced a progressive proliferation of tight junctions in the rat liver. This system proved to be rapid and highly reproducable and affords the opportunity for tracing the fate of tight junctions in freeze-fracture replicas, facilitating investigations on their formation and functi...

journal_title：European journal of cell biology

authors： Robenek H,Themann H,Ott K

更新日期：1979-10-01 00:00:00

abstract：:Stereo immunofluorescence microscopy avoids the problem of juxtaposition of structures often encountered in normal fluorescence microscopy. The procedure has been used in conjunction with antibodies against microfilament associated proteins to reveal the arrangement of microfilaments in a rat mammary cell line both in...

journal_title：European journal of cell biology

authors： Osborn M,Weber K

更新日期：1979-10-01 00:00:00

abstract：:The action of a series of chelators, cryptates and ionophores on microfibril deposition and the presence of cortical microtubules in Oocystis solitaria has been investigated. Having a potent inhibitory effect on cellulose synthesis were the calcium ionophores A 23187 and X-537A, the flourescent calcium chelator chloro...

journal_title：European journal of cell biology

authors： Quader H,Robinson DG

更新日期：1979-10-01 00:00:00

abstract：:In giant neurons of whip spider legs several filament types are detectable: filaments of 5 to 6 nm thickness as dense masses within the soma of the neuron, an intermediate-sized filament type limited to the dendritic processes forming irregularly wound bundles and finally twisted double filaments in the soma as well a...

journal_title：European journal of cell biology

authors： Foelix RF,Hauser M

更新日期：1979-08-01 00:00:00

abstract：:Sarcoplasmic reticulum fragments capable of accumulating calcium were isolated from rat skeletal muscle by differential and sucrose gradient centrifugation. The ability of these fragments to accumulate calcium was impaired by adding 2,2-bis-(p-chlorophenyl)-1,1,1-trichloroethane (DDT) to the assay medium at concentrat...

journal_title：European journal of cell biology

authors： Elder JH,Morré DJ,Yunghans WN

更新日期：1979-08-01 00:00:00

abstract：:Conventional fixation with glutaraldehyde fixatives at pH 7.4 did not preserve/promote thick filaments in Bufo smooth muscle. However, if pH was lowered to 6.0, thick filaments were present and addition of dimethyl sulfoxide (DMSO) led to a greater number of thick filaments. Stretch alone had little effect on the pres...

journal_title：European journal of cell biology

authors： Bagby RM,Fisher BA

更新日期：1979-06-01 00:00:00

abstract：:The fine structure of a joint receptor (R10) in a spider leg (Zygiella x-notata) was examined with light and electron microscopy. The R10 receptor consists of a compact ganglion which is situated near the dorsal joint membrane of the femur/patella joint. Each of the ten sensory cells comprising the ganglion sends one ...

journal_title：European journal of cell biology

authors： Foelix RF,Choms A

更新日期：1979-06-01 00:00:00